The neomycin resistance encoding RNA is highly enriched in the resulting EVs compared with their cells of origin. The use of the BOGOTA construct results in a selective packaging of the reporter gene into EVs. Results: Preliminary studies indicate that the transfer of retroviral elements is successful between different cell lines. Expression of neomycin is used to select for cell recipients and the initial confirmation of integration of the transferred retroviral element into the genome of the recipient. We have screened several cell lines for the presence of HERVs. Methods: The assay is based on the BOGOTA construct (Contreras-Galindo et al., 2015), which includes parts of the HERV-K113 packaging signal, and is selected through a neomycin reporter gene. Hence, we are now testing whether a high prevalence of the retrotransposon-like elements known as human endogenous retroviruses (HERVs) in tumour cell lines leads to selective packaging and transfer of exRNA through EVs. Furthermore, the production of EVs seems to share resemblance with retroviral particle assembly, thus making retroviral-like particles a component of the EV contingent. Interestingly, retrotransposon elements are highly enriched in tumour extracellular vesicles (EVs) compared with their cells of origin and have been shown to be transferred in vitro from medulloblastoma cells to normal human umbilical vein endothelial cells (Balaj et al., 2011). Scattered throughout the genome, these elements are capable of amplifying themselves as well as changing their position through RNA intermediates. Introduction: Retrotransposons are remnants of ancient viral infections that infected the genome of our primate lineage through infection of germ cells. Breakefield 1ġDepartment of Neurology and Radiology and Program in Neuroscience, Massachusetts General Hospital and Harvard Medical School, Boston, Maine, USA 2Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA Friis 1, Leonora Balaj 1, Romy Verschoor 1, Rafael Contreras-Galindo 2, Shilpa Prabhakar 1, David M. Special Achievement Award, Wrap-up Sessions, Oral and Poster AwardsĬlosing Remarks and Announcement of ISEV2017 Plenary Session 4 with Featured Abstracts ISEV2016 Poster Session 7 - Late Breaking Posters Session 2
Poster Session 6 - Novel Technical Developments in EV Characterization Poster Session 5 - MSCs & Tissue Regeneration and Morphogenesis
Poster Session 4 - EVs in the Tumor Microenvironment Poster Session 3 - EVs and the Immune System Poster Session 8 - Late Breaking Poster Session 1ĮVs as Modulators of Drug Resistance and Tumor MetastasisĮV-Associated Versus Soluble Functional MoleculesĮVs in Acute and Chronic Inflammatory DiseaseĮVs in Immune Modulation in Bacterial and Parasitic InfectionsĮVs in Cellular Differentiation and Development Poster Session 7 - EV Characterization from Different Sources or Different Subtypes Poster Session 5 - EVs as Cancer Biomarkers Poster Session 3 - EVs in Cardiovascular Disease and Coagulation Poster Session 2 - EVs in Cardiovascular Disease Poster Session 1 - EVs, Microbes & Cross-species Communication Meet the National and International SocietiesĮV Heterogeneity: Can we Overcome the Confusion? Poster Session 8 - EVs in Prostate Cancer Poster Session 6 - EV Proteomics & Lipidomics Poster Session 5 - EVs in Acute and Chronic Inflammation Poster Session 4 - EVs as Drug Delivery System for Antitumoral Therapies & Vaccination Poster Session 3 - Analysis of EVs in Body Fluids Poster Session 2 - EVs in Tumor Metastasis Poster Session 1 - Cell Biology of EVs: Biogenesis and Transfer Lyden (USA)ĮV Isolation, Characterization and Stem Cellsīiotech Sponsored session: CARIS Life SciencesĮVs in Metabolic Syndrome, Fatty Liver Disease and Cancer
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